Journal: Toxicology Research

Article Title: Silica nanoparticles induce unfolded protein reaction mediated apoptosis in spermatocyte cells

doi: 10.1093/toxres/tfaa036

Figure Lengend Snippet: The changes of unfolded protein reaction in spermatocyte cells after exposure to SiNPs for 1, 10, 20 and 30 generations. Notes: A: Protein bands of GRP-78/p-PERK/IRE1α/ATF6. B: The proteins expression of GRP-78/p-PERK/IRE1α/ATF6. SiNPs hasten the development of UPR-related proteins GRP-78/p-PERK/IRE1α/ATF6. *P < 0.05 represents significantly difference between the control group and the 5 μg/ml SiNPs group in the same generation.

Article Snippet: After sealing with TBST and BSA for 1.5 h, the NC membranes were incubated with p-PERK, GRP-78, IRE1α, ATF6 (1:500, rabbit antibodies, Boster Biological Technology Co., Ltd, China) and Caspase 3 (1:1000, rabbit antibodies, Cell Signaling Technology, Beverly, MA, USA) at room temperature for 1 h and overnight at 4°C.

Techniques: Expressing, Control